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Effect of AAV-SNCA on levels of DA, as well as the expression levels of protein in both the VTA and the NAc. ( A-D ) WB analysis of α-syn, p-α-syn, and TH in the VTA ( n = 5). ( E ) DA levels in the VTA and the NAc by ELISA ( n = 6; VTA: left Y axis, NAc: right Y axis). ( F-I ) WB analysis of SNAP-25, <t>PSD95,</t> and DAT in the NAc ( n = 5). ( J , K ) WB analysis of D1R and D2R in NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. AAV-NC1 (one-way ANOVA with Tukey’s test).
Postsynaptic Density Protein 95 Psd95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/postsynaptic density protein 95 psd95/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
postsynaptic density protein 95 psd95 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
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Effect of AAV-SNCA on levels of DA, as well as the expression levels of protein in both the VTA and the NAc. ( A-D ) WB analysis of α-syn, p-α-syn, and TH in the VTA ( n = 5). ( E ) DA levels in the VTA and the NAc by ELISA ( n = 6; VTA: left Y axis, NAc: right Y axis). ( F-I ) WB analysis of SNAP-25, <t>PSD95,</t> and DAT in the NAc ( n = 5). ( J , K ) WB analysis of D1R and D2R in NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. AAV-NC1 (one-way ANOVA with Tukey’s test).
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Effect of AAV-SNCA on levels of DA, as well as the expression levels of protein in both the VTA and the NAc. ( A-D ) WB analysis of α-syn, p-α-syn, and TH in the VTA ( n = 5). ( E ) DA levels in the VTA and the NAc by ELISA ( n = 6; VTA: left Y axis, NAc: right Y axis). ( F-I ) WB analysis of SNAP-25, <t>PSD95,</t> and DAT in the NAc ( n = 5). ( J , K ) WB analysis of D1R and D2R in NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. AAV-NC1 (one-way ANOVA with Tukey’s test).
Antibodies Against Postsynaptic Density Protein 95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against postsynaptic density protein 95/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
antibodies against postsynaptic density protein 95 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

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Effect of AAV-SNCA on levels of DA, as well as the expression levels of protein in both the VTA and the NAc. ( A-D ) WB analysis of α-syn, p-α-syn, and TH in the VTA ( n = 5). ( E ) DA levels in the VTA and the NAc by ELISA ( n = 6; VTA: left Y axis, NAc: right Y axis). ( F-I ) WB analysis of SNAP-25, PSD95, and DAT in the NAc ( n = 5). ( J , K ) WB analysis of D1R and D2R in NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. AAV-NC1 (one-way ANOVA with Tukey’s test).

Journal: Scientific Reports

Article Title: GDNF attenuates a-synuclein aggregation-induced damage to VTA-NAc dopaminergic transmission and alleviates depression-like behaviors in mice

doi: 10.1038/s41598-025-16556-7

Figure Lengend Snippet: Effect of AAV-SNCA on levels of DA, as well as the expression levels of protein in both the VTA and the NAc. ( A-D ) WB analysis of α-syn, p-α-syn, and TH in the VTA ( n = 5). ( E ) DA levels in the VTA and the NAc by ELISA ( n = 6; VTA: left Y axis, NAc: right Y axis). ( F-I ) WB analysis of SNAP-25, PSD95, and DAT in the NAc ( n = 5). ( J , K ) WB analysis of D1R and D2R in NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. AAV-NC1 (one-way ANOVA with Tukey’s test).

Article Snippet: The membranes were closed for 2 h at room temperature (20–25 °C) with 5% skimmed milk, which subsequently, were incubated overnight at 4 °C with specified primary antibodies (α-syn, Abcam, Cat# ab138501; TH, Santa, Cat# sc-374048; p-α-syn, Thermo Fisher Scientific, Cat# PA5-37740; synaptosomal-associated protein 25 (SNAP-25), Santa, Cat# sc-20038; postsynaptic density protein 95 (PSD95), CST, Cat# 3450 S; D2R, Santa, Cat# sc-5303; D1R, CST, Cat# #79777T; DAT, Santa, Cat# sc-32259; GDNF, Affinity, Cat# DF7727; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), huabio, Cat# ET1601-4; β-actin, Proteintech, Cat# 66009-l-lg).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

The overexpression of GDNF counteracted the impact of α-syn aggregation on protein expression levels in both the VTA and NAc. ( A-D ) WB analysis of p-α-syn, GDNF and TH in the VTA ( n = 5). ( E-I ) WB analysis of SNAP-25, PSD95, D2R and D1R in the NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way ANOVA with Tukey’s test).

Journal: Scientific Reports

Article Title: GDNF attenuates a-synuclein aggregation-induced damage to VTA-NAc dopaminergic transmission and alleviates depression-like behaviors in mice

doi: 10.1038/s41598-025-16556-7

Figure Lengend Snippet: The overexpression of GDNF counteracted the impact of α-syn aggregation on protein expression levels in both the VTA and NAc. ( A-D ) WB analysis of p-α-syn, GDNF and TH in the VTA ( n = 5). ( E-I ) WB analysis of SNAP-25, PSD95, D2R and D1R in the NAc ( n = 5). Data: Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way ANOVA with Tukey’s test).

Article Snippet: The membranes were closed for 2 h at room temperature (20–25 °C) with 5% skimmed milk, which subsequently, were incubated overnight at 4 °C with specified primary antibodies (α-syn, Abcam, Cat# ab138501; TH, Santa, Cat# sc-374048; p-α-syn, Thermo Fisher Scientific, Cat# PA5-37740; synaptosomal-associated protein 25 (SNAP-25), Santa, Cat# sc-20038; postsynaptic density protein 95 (PSD95), CST, Cat# 3450 S; D2R, Santa, Cat# sc-5303; D1R, CST, Cat# #79777T; DAT, Santa, Cat# sc-32259; GDNF, Affinity, Cat# DF7727; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), huabio, Cat# ET1601-4; β-actin, Proteintech, Cat# 66009-l-lg).

Techniques: Over Expression, Expressing